Evaluation of functional gene enrichment in a soil metagenomic clone library.
نویسندگان
چکیده
We evaluated the use of mixed oligonucleotide probes hybridized to metagenomic clones spotted on high density membranes. The pooled probes included oligonucleotides designed for genes associated with denitrification, antibiotic resistance, and dehalogenation among others. Pyrosequence comparison between the clones and the original DNA demonstrated the utility of clone screening with pooled probes.
منابع مشابه
Size Does Matter: Application-driven Approaches for Soil Metagenomics.
Metagenomic analyses can provide extensive information on the structure, composition, and predicted gene functions of diverse environmental microbial assemblages. Each environment presents its own unique challenges to metagenomic investigation and requires a specifically designed approach to accommodate physicochemical and biotic factors unique to each environment that can pose technical hurdle...
متن کاملIdentification of novel glycosyl hydrolases with cellulolytic activity against crystalline cellulose from metagenomic libraries constructed from bacterial enrichment cultures
To obtain cellulases that are capable of degrading crystalline cellulose and cedar wood, metagenomic libraries were constructed from raw soil sample which was covered to pile of cedar wood sawdust or from its enrichment cultures. The efficiency of screening of metagenomic library was improved more than 3 times by repeating enrichment cultivation using crystalline cellulose as a carbon source, c...
متن کاملMetagenomic Analysis of a Southern Maritime Antarctic Soil
Our current understanding of Antarctic soils is derived from direct culture on selective media, biodiversity studies based on clone library construction and analysis, quantitative PCR amplification of specific gene sequences and the application of generic microarrays for microbial community analysis. Here, we investigated the biodiversity and functional potential of a soil community at Mars Oas...
متن کاملNovel Inorganic Pyrophosphatase from Soil Metagenomic and Family and Subfamily Prediction
Inorganic pyrophosphatase (PPase) is widely studied, to be extremely important for survival of plants and microorganisms. PPases catalyze an essential reaction the hydrolysis of inorganic pyrophosphate (PPi) to inorganic phosphate (Pi). Studies involving the mechanism of PPase were performed in microorganisms culture. We didn’t found reports of PPase derived from soil metagenomic libraries. Soi...
متن کاملIdentification of a complete methane monooxygenase operon from soil by combining stable isotope probing and metagenomic analysis.
Stable isotope probing (SIP) allows the isolation of nucleic acids from targeted metabolically active organisms in environmental samples. In previous studies, DNA-SIP has been performed with the one-carbon growth substrates methane and methanol to study methylotrophic organisms. The methylotrophs that incorporated the labelled substrate were identified with polymerase chain reaction and sequenc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of microbiological methods
دوره 76 1 شماره
صفحات -
تاریخ انتشار 2009